ANALISA BEBERAPA PARAMETER MOTILITAS SPERMATOZOA PADA BERBAGAI BANGSA SAPI MENGGUNAKAN COMPUTER ASSISTED SEMEN ANALYSIS (CASA)

Sarastina Sarastina, T. Susilawati, G. Ciptadi

Abstract


ABSTRAK
Tujuan Penelitian ini adalah untuk mempelajari 18 parameter motilitas
spermatozoa menggunakan metode CASA serta motilitas progresif secara visual
dibandingkan ddengan metoda CASA. Materi adalah semen segar dari 8 bangsa sapi
yaitu Limousin, Bali Madura, Simental, Brahman, dan Ongole. Sata dianalisa dengan
analisis varian, sementara data motilitas progresif diuji t-test. Hasil menunjukkan
bahwa motilitas progresif menggunakan CASA tidak berbeda nyata (P>0.05) diantara
6 bangsa sapi. Sementara itu parameter motilitas hiperaktif menggunakan CASA tidak
berbeda nyata (P>0.05) kecuali on linier, non limear, juga parameter: DCL, DAP,
DSL, VCL, VAP, VSL, LIN< STR< WOB, ALH, AOH adalah berbeda nyata
(P<0.05) diantara bangsa sapi. Uji t pada motilitas progresif menunjukkan tidak ada
beda nyata (P>0.05) antara CASA dengan visula.
Kata kunci: Motilitas, CASA, spermatozoa, sapi.
ABSTRACT
The aims of this research were to determine 18 parameters spermatozoa
motility using CASA method and to determine spermatozoa progressive motility using
visual assessment and CASA. The material of this research were bull fresh semen
from 6 breeds, there were Limmousin, Bali, Madura, Simmental, Brahman and
Ongole which owned by National Artificial Insemination Center Singosari. Data were
collected from CASA method of 18 parameters sperm motility and visual assessment
method of progressive sperm motility. Data were analyzed by variance and factor
analysis, and progressive motility using visual and CASA were analyzed by paired TTest.
The result showed that motility and progressive motility using CASA was no
significant different (P>0.05) for six breeds. There were no significant different
(P>0.05) on hyperactive parameter using CASA, but on linear, non linear, curve
linear and cell detail parameter : DCL, DAP, DSL, VCL, VAP, VSL, LIN, STR,
WOB, ALH, AOH were significant different (P<0.05) among breeds. Analysis using
paired T-test of progressive motility assessment was no signifgicant different between
CASA and visual microscopic (P>0.05).

Key words : Motility, spermatozoa, CASA, breeds


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