PENGARUH LAMA SIMPAN SEMEN TERHADAP KUALITAS SPERMATOZOA AYAM KAMPUNG DALAM PENGENCER RINGER’S PADA SUHU 4 C

Authors

  • D.R. Danang Bagian Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya
  • N. Isnaini Bagian Produksi Ternak Fakultas Peternakan Universitas Brawijaya Malang
  • P. Trisunuwati Bagian Produksi Ternak Fakultas Peternakan Universitas Brawijaya Malang

Abstract

Tujuan penelitian adalah untuk mengetahui pengaruh lama simpan semen pada
suhu 40C terhadap kualitas spermatozoa ayam kampung dalam pengencer Ringer
solution. Hasil penelitian diharapkan berguna sebagai informasi tentang waktu optimal
lama simpan spermatozoa ayam kampung dalam pengencer Ringer solution pada suhu
40C sehingga dapat menunjang keberhasilan IB. Materi yang digunakan adalah semen
ayam Kampung berasal dari empat ekor ayam kampung jantan berumur 1 - 1,5 tahun,
bobot badan ± 2,2 kg. Penelitian dilakukan menggunakan metode percobaan dengan
perlakuan larutan pengencer Ringer’s pada suhu 4 0C menggunakan enam waktu
pengamatan yang berbeda yaitu pada jam ke 0, 6, 12, 18, 24 dan 30. Ulangan 10
kali. Data yang diperoleh dianalisis dengan analisis ragam. Rancangan yang
digunakan adalah Rancangan Acak Lengkap (RAL), apabila hasil yang diperoleh
menunjukkan perbedaan pengaruh yang nyata (P<0,05) atau sangat nyata (P<0,01)
maka dilanjutkan dengan Uji Beda Nyata Terkecil (BNT). Parameter yang diamati:
motilitas individu, viabilitas dan abnormalitas spermatozoa. Hasil Penelitian
menunjukkan bahwa lama simpan pada suhu 40C memberikan pengaruh yang sangat
nyata (P<0.01) terhadap kualitas spermatozoa ayam kampung yang meliputi motilitas
individu, persentase viabilitas, dan persentase abnormalitas dalam pengencer Ringer.
Kualitas semen dapat dipertahankan sampai 18 jam yang memiliki kualitas semen yaitu
: motilitas individu 47 + 5.87, viabilitas 69.4 + 3.34 dan abnormalitas 15 + 0.82.
Disimpulkan bahwa kualitas semen ayam Kampung yang diencerkan dengan Ringer’s
yang disimpan pada suhu 4 0C mengalami penurunan secara bertahap dan dapat
digunakan untuk IB dalam waktu tidak lebih dari 18 jam setelah penampungan.
Kata kunci : kualitas spermatozoa ayam Kampung , pengencer Ringer’s , suhu 4 0C
THE EFFECT OF STORAGE TIME ON NATIVE CHIKENS
SPERMATOZOA QUALITY BY RINGER SOLUTION DILUENT IN 4 0C
TEMPERATURE
ABSTRACT
The aim of the study was to find out endurance of Kampung chicken semen quality
diluted by Ringer’s of various storage time at 4 0C. The result of the study can be used
as infomation resource about the optimal time to store the Native chicken spermatozoa
with Ringer’s diluent at 4 0C to support the successful of Artificial Insemination
program. The research material was semen of Native chicken from four rooster from 1
-1.5 years old and about ± 2.2 kg body weight. The research was design by
Completely Randomized Design (CRD) with 6 treatment and 10 replication, if therewere significant influence, it would be tested by least significant Different Test. The
variable’s observed were individual motility, sperm viability, and sperm abnormality.
The result showed that the quality of semen in Ringer’s diluter significantly influenced
by long storage at 4 0C temperature. Semen quality can be used up to 18 hours with:
47 ± 5.87 individual motility, 69.4 ± 3.34 percentage of viability, and 15 ± 0.82
abnormalities. The conclution is the quality of native chicken semen decrease
gradually in the ringer solution at 4oC storage.To support the successful of AI, it will
be better if the semen of Native chicken diluted by Ringer’s and stored not more than
18 hours at 4 0C temperature.

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How to Cite

Danang, D., Isnaini, N., & Trisunuwati, P. (2012). PENGARUH LAMA SIMPAN SEMEN TERHADAP KUALITAS SPERMATOZOA AYAM KAMPUNG DALAM PENGENCER RINGER’S PADA SUHU 4 C. TERNAK TROPIKA Journal of Tropical Animal Production, 13(1), 47–57. Retrieved from https://ternaktropika.ub.ac.id/index.php/tropika/article/view/163

Issue

Vol. 13 No. 1 (2012): Ternak Tropika

Section

Articles

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