Analisis Profil Protein Darah Anak Kambing Peranakan Etawah Dengan Pemberian Pakan Substitusi Susu Sapi

Teguh Wicaksono, Gatot Ciptadi, Tri Eko Susilorini


The objective of this study is to determine the protein profile of pre-weaning kids fed with cow's milk as a substitute for dam’s milk. The materials used were 18 Etawah Descendant (PE) kids born the twin at the age of 5-13 days from 3-4-year-old dams. This experimental design was a completely randomized design with three treatments with six replications per treatment, namely the control (T0) fed 100% goat’s milk, treatment 1 (T1) fed 50% goat’s milk and 50% cow’s milk, treatment 2 (T2) fed 100% cow’s milk. The protein profile serum was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method, 12,5% of the resolving gel and 3% of the stacking gel were used. The protein profile of the 5-14 days old PE kids were 19 protein bands with the molecular weight ranging from 15-160 kDa. The kids fed with 100% goat milk (T0) and those substituted by 50% cow's milk (T1), it was produced 19 protein bands with molecular weights ranging from 15 kDa to 155 kDa, while those fed with 100 % cow's milk (T2), it was produced 17 protein bands with molecular weights ranging from 13 kDa to 160 kDa. It can be concluded that the dam's milk substitute using cow's milk at the 50% level does not affect the blood protein profile of goat kids, while the 100% substitute produces the different number and types of protein


Proteomic; Molecular weight; Goat kids; Electrophoretic

Full Text:



Castellano L. E. H., A. Morales-delaNues, D. Sanches-Macias, I. Moreno-Indias, A. Torres, J. Capote, A. Argüello dan N. Castro. 2015. The effect of colostrum source (goat vs. sheep) and timing of the first colostrum feeding (2 h vs. 14 h after birth) on body weight and immune status of artificially reared newborn lambs. J. Dairy Sci. 98 No. 1:204–210.

Otzule L. dan A. Ilgaža. 2014. Goat Kids Stomach Orphological Development Depending on The Milk Type. Research for Rural Development. 1:185-189.

Sagitarini D., S. Utami dan T. Y. Astuti. 2013. Kadar protein dan nilai viskositas susu kambing sapera di cilacap dan bogor. Jurnal Ilmiah Peternakan. 1(3):1057-1063.

Jooyandeh H. dan A. Aberoumand. 2010. Phsyco-chemical, nutritional, heat treatment effects and dairy products aspects of goat and sheep milks. World Appl. Sci. J. 11(11): 1316-1322.

Abbas H. M., F. A. M. Hassan, M. A. M. A. El-Gawad dan A. K. Enab. 2014. Physicochemical characteristics of goat’s milk. Life Sci J. 11(1): 307-317.

Jenness R.. 1980. Composition and characteristics of goats milk. J. Dairy Sci. 63:1605- 1630.

Supriyati. 2012. Pertumbuhan kambing Peranakan Etawah prasapih yang diberi susu pengganti. JITV. 17(2):142-151.

Sultana N., S. M. J. Hossain, S. Sultana dan M. R. Hassan. 2014. Development of milk replacer for rearing kids. The Bangladesh Veterinarian. 31(1):46 – 54.

Chaniago T. D. dan Hastono. 2001. Pertumbuhan pra-sapih anak kambing peranakan etawah yang diberi susu pengganti. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner p: 241-246,

Lepczyński A., A. Herosimczyk, M. Ożgo dan W. F. Skrzypczak. 2015. Feeding milk replacer instead of whole milk affects blood plasma proteome and lipid profile in preruminant calves. Polish Journal of Veterinary Sciences. 18(1):91–99,

Lima A. L., D. B. Moretti, W. M. Nordi, P. Pauletti, I. Susin dan R. Machado-Neto. 2013. Eletrophoretic profile of serum proteins of goat kids fed with bovine colostrum in natura and lyophilized. Small Ruminant Research. 113: 278 – 282.

Fatchiyah, L. A. Arumingtyas, S. Widyarti and S. Rahayu. 2011. Biologi Molekuler, Prinsip Dasar Analis. Erlangga. Jakarta.

Anous M. R., M. A. Rashed, H. R. Motaoa, M. H. Sadek, Y. M. Saad, M. A. Osman dan E. M. Shath. 2008. Identification of fecundity gene in Egyptian goats using genetic markers. Egypt. J. Genet. Cytol. 37: 83-94.

Saut J. P. E., R. M. de Souza, D. B. Birgel, F. C. Pogliani, C. Z. Cavalcante, S. I. Miyashiro, J. J. Fagliari dan E. H. B. Junior. 2009. Influence of puerperium on the serum proteins of Saanen goats, obtained by the analysis of electrophoresis on sodium dodecyl sulphate-polyacrylamide gel. Semina: Ciências Agrárias, Londrina. 30(3): 661-670.

Prata A. S. dan V. C. Sgarbieri. 2008. Composition and physicochemical properties of two protein fractions of bovine blood serum. Ciênc. Tecnol. Aliment., Campinas. 28(4): 964-972.

Mordacq J. C. dan R. W. Ellington. 1994. Polyacrylamide gel electrophoresis (PAGE) of blood proteins. Pages 15-44, in Tested studies for laboratory teaching, Volume 15 (C. A. Goldman, Editor). Proceedings of the 15th Workshop/ Conference of the Association for Biology Laboratory Education (ABLE).

Moreno-Indias I., A. D. Dodds, A. Argüello, N. Castro and R. B. Sim. 2012. The complement system of the goat: Haemolytic assays and isolation of major protein. BMC Veterinary Research. 8:91.

Aminlari M., S. Gholami, Gh. Parhizgari dan S. Ranjbar. 2006. Comparison of optic lens proteins among animals at different stages of development. Iranian Journal of Veterinary Research. 7(1):1-7.

Tóthová C., C. Nagy dan G. Kováč. 2014. Changes in the Concentrations of Serum Protein Fractions in Calves with Age and Nutrition. Italian Journal of Animal Science. 13(1):107-111.

Putra G. W. K., U. Lestari and S. E. Rahayu. 2014. Analisis protein membran spermatozoa kambing peranakan etawa, kambing boer, dan kambing kacang sebagai pendekatan kekerabatan.

Torbati F.M., A. Kocer, A. Auguste, L. Renault, G. Charpigny, E. Pailhoux and M. Pannetier. 2010. A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long-lasting SRY expression. Developmental Dynamic. 239(12):24-35.

Palupi E. P., A. Jayanegara, B. Setiawan and A. Sulaeman. 2012. Comparison of Nutrition Quality between Cow and Goat Dairy Products: A Meta-analysis. Proceedings of the 1st Asia Dairy Goat Conference, Kuala Lumpur, Malaysia, pp.162-164, 9–12 April 2012.

Richardson C. W. 2015. Let’s Compare Dairy Goats and Cows. Oklahoma Cooperative Extension Service. Division of Agricultural Sciences and Natural Resources.

Salem S. A., E. I. El-Agamy, F. A. Salama dan N. H. Abo-Soliman. 2009. Isolation, molecular and biochemical characterization of goat milk casein and its fractions. Tropical and Subtropical Agroecosystems. 11: 29 – 35.

Oztabak K. dan A. Ozpinar. 2006. Growth performance and metabolic profile of chios lambs prevented from colostrum intake and artificially reared on a calf milk replacer. Turk. J. Vet. Anim. Sci. 30:319-324.

Rule D. C., G. E. Moss, G. D. Snowder dan N. E. Cockett. 2002. Adipose Tissue Lipogenic Enzyme Activity, Serum IGF-I, and IGF-Binding Proteins in the Callipyge Lamb. Sheep & Goat Research Journal. 17:39-46.

Rodríguez C. N., J. Castro, A. Capote, I. Morales-dela Nuez, Moreno-Indias, D. Sánchez-Macías, dan A. Argüello. 2009. Effect of colostrums immunoglobulin concentration on immunity in Majorera goat kids. J. Dairy Sci, 92 (4): 1696–1701

Publication ID


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.