Single Nucleotide Polymorphims (snps) Identification of Inhibin Sub Unit-α (inha) Gene on Madura Bulls

Authors

  • Irida Novianti Faculty of Animal Science, Universitas Brawijaya, Kota Malang, Jawa Timur, 65145, Indonesia
  • Chairdin Dwi Nugraha Faculty of Animal Science, Universitas Brawijaya, Kota Malang, Jawa Timur, 65145, Indonesia
  • Rafika Febriani Putri Faculty of Animal Science, Universitas Brawijaya, Kota Malang, Jawa Timur, 65145, Indonesia
  • Ahmad Furqon Faculty of Animal Science, Universitas Brawijaya, Kota Malang, Jawa Timur, 65145, Indonesia
  • Wike Andre Septian Faculty of Animal Science, Universitas Brawijaya, Kota Malang, Jawa Timur, 65145, Indonesia
  • S. Rahayu Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Kota Malang, Jawa Timur, 65145, Indonesia
  • V. M. A. Nurgiartiningsih Faculty of Animal Science, Universitas Brawijaya, Kota Malang, Jawa Timur, 65145, Indonesia
  • Suyadi Suyadi Faculty of Animal Science, Universitas Brawijaya, Kota Malang, Jawa Timur, 65145, Indonesia

DOI:

https://doi.org/10.21776/ub.jtapro.2021.022.01.10

Keywords:

INHA, polymorhphisms, heterozygosity, Madura bulls

Abstract

INHA gene is a gene that is suggested to have role in reproductive system. Single Nucleotide Polymorphisms (SNPs) of Madura Bulls were identified in this study. Polymerase Chain Reactions (PCR) was used to amplify INHA gene region and MEGA 7 program was utilized to align the amplified region sequences with sequence from Ensembl database. Four SNPs found in INHA and they are located at the first exon. Two SNPs were misssense mutations that causing the substitution of amino acid leucine21 by proline, and amino acid valine63 by methionine and the other two SNPs were synonymous mutation. One of the synonymous SNPs was a novel mutation. Based on those identified SNPs, they could be suggested as potential candidate markers of reproduction traits for Madura bulls. Moreover, through heterozygosity value from the observed bulls, it was indicated that the genotype was varied in population. Therefore a molecular selection program could be designed to determine the Madura superior bull.

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Published

2021-06-30

How to Cite

Novianti, I., Nugraha, C. D., Putri, R. F., Furqon, A., Septian, W. A., Rahayu, S., Nurgiartiningsih, V. M. A., & Suyadi, S. (2021). Single Nucleotide Polymorphims (snps) Identification of Inhibin Sub Unit-α (inha) Gene on Madura Bulls. TERNAK TROPIKA Journal of Tropical Animal Production, 22(1), 77–81. https://doi.org/10.21776/ub.jtapro.2021.022.01.10

Issue

Vol. 22 No. 1 (2021): TERNAK TROPIKA Journal of Tropical Animal Production

Section

Articles

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